1 ml 4. 1 ml 1. Let’s take an example of two products — DSL Service and DSL Modem. The Omniscript Reverse Transcription (RT) Kit is specially designed for reverse transcription with any amount of RNA from 50 ng to 2 μg per reaction. For reverse transcription of 50 ng to 2 micro g RNA per reaction for end-point PCR. A high affinity for RNA allows Omniscript Reverse Transcriptase to provide superior performance compared with other reverse transcriptases, delivering higher sensitivity in RT-PCR, even with low. Simply add primers for fast and easy cDNA synthesis. 1 ml 1. CNV qPCR Assays & Arrays;. For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCR(RT-PCR) RNA was extracted from cultured macrophages with RNeasy Mini Kit (Qiagen, Hilden, Germany) and made into cDNA with Omniscript RT Kit (Qiagen) according to the manufac-turer’s instructions. The total volume per reaction is set to 20 μl. OmniStudio Standard. For 50 reverse-transcription reactions: 200 units Omniscript Reverse Transcriptase, 150 µL 10 × Buffer RT, 100 µL dNTP Mix (contains 5 mM each dNTP), 1. CNV qPCR Assays & Arrays;. Each experimental condition was run in 10 RT replicates unless stated otherwise. For 50 reverse-transcription reactions: 200 units Omniscript Reverse Transcriptase, 150 µl 10x Buffer RT, 100 µl dNTP Mix (contains 5 mM each dNTP), 1. A high affinity for RNA allows Omniscript Reverse Transcriptase to provide superior performance compared with other reverse transcriptases, delivering higher sensitivity in RT-PCR, even with low. 1 ml 4. It is a quantitative technique used to estimate gene copies. ZERO BIAS - scores, article reviews, protocol conditions and morePeripheral blood of individual 4 was collected into PAXgene Blood RNA tubes (PreAnalytiX), and RNA was isolated using the PAXgene Blood RNA Kit (Qiagen). For 50 reverse-transcription reactions: 200 units Omniscript Reverse Transcriptase, 150 µl 10x Buffer RT, 100 µl dNTP Mix (contains 5 mM each dNTP), 1. OmniStudio Standard. Technical Service; Customer Care . 1 ml 4. Migrate to Standard OmniStudio. Both enzymes exhibit a high affinity for RNA, facilitating transcription through secondary structures that may inhibit other reverse transcriptases. Workflow for Configuring OmniAnalytics for a Third-Party Analytics. 4 Omniscript Reverse Transcription Handbook 10/2010 Kit Contents Omniscript RT Kit (10) (50) (200) Catalog no. Quantitative real-time PCR was performed on a QuantStudio 6 Pro real-time PCR system and Taqman Universal PCR Master Mix according to the manufacturer instructions. 205110 205111 205113 Number of reactions* 10 50 200 Omniscript Reverse Transcriptase 40 units 200 units 800 units Buffer RT, 10x 30 µl 150 µl 4 x 150 µl dNTP Mix, 5 mM each 20 µl 100 µl 4 x 100 µl RNase-Free Water 1. Simply add primers for fast and easy cDNA synthesis. 2800. Omniscript Reverse Transcriptase is a recombinant heterodimeric enzyme expressed in E. Omniscript Reverse Transcription Kit (Qiagen). g. For reverse transcription of 50 ng to 2 micro g RNA per reaction for end-point PCR. 31 Australia QIAGEN Pty Ltd PO Box 641 DONCASTER VIC 3108 Orders 03-9840-9800 Fax 03-9840-9888 Technical 1-800-243-066 Belgium QIAGEN Benelux B. For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCROmniscript® Reverse TranscriptionFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCR3 min read. Ordering Support. For the reverse-transcription step, both Omniscript and Sensiscript are functionalized with an RT-blocker, which keeps them in an inactive state and prevents nonspecific transcription at ambient temperature. One-Step RT-PCR; Reverse Transcription & cDNA Synthesis; Real-Time PCR Enzymes & Kits. For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRThe Omniscript Reverse Transcription (RT) Kit is specially designed for reverse transcription with any amount of RNA from 50 ng to 2 μg per reaction. 0 µl of 10 µM H-T11C (final concentration of. Qiagen omniscript reverse transcription rt kit Omniscript Reverse Transcription Rt Kit, supplied by Qiagen, used in various techniques. Probe-Based One-Step qRT-PCR; Probe-Based qPCR; Reverse Transcription & cDNA Synthesis for qPCR; SYBR Green- or Dye-Based One-Step qRT-PCR; SYBR Green- or Dye-Based qPCR; qPCR Assays & Instruments. Specific genes have been used as baseline controls to. After the cDNA was diluted 1:3 in nuclease-free water, qRT-PCR was performed using the custom-designed primers (Biomers, Ulm, Germany) in Table 1 and the Platinum SYBR Green qPCR SuperMix-UDG kit (Invitrogen), or TaqMan Gene Expression Assays and. This also inactivates the reverse transcriptases. 205110 205111 205113 Number of reactions* 10 50 200 Omniscript Reverse Transcriptase 40 units 200 units 800 units Buffer RT, 10x 30 µl 150 µl 4 x 150 µl dNTP Mix, 5 mM each 20 µl 100 µl 4 x 100 µl RNase-Free Water 1. The one-step format prevents contamination and allows for higher sensitivity. 205110 205111 205113 Number of reactions* 10 50 200 Omniscript Reverse Transcriptase 40 units 200 units 800 units Buffer RT, 10x 30 µl 150 µl 4 x 150 µl dNTP Mix, 5 mM each 20 µl 100 µl 4 x 100 µl RNase-Free Water 1. For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRQiagen omniscript rt kit Omniscript Rt Kit, supplied by Qiagen, used in various techniques. Tie a Picklist to Salesforce. One-Step RT-PCR; Reverse Transcription & cDNA Synthesis; Real-Time PCR Enzymes & Kits. SuperScript III RT offers higher cDNA yields, improved cDNA lengths, improved efficiency on GC-rich. Principle Omniscript Reverse Transcriptase (RT) has a high affinity for RNA, which enables efficient and sensitive reverse transcription of any template, leading to high yields of cDNA. Direct quantification of six different targets in human total RNA using GoTaq® 2-Step RT-qPCR System. reverse transcription of RNA amounts greater than 50 ng, and Sensiscript is optimized for use with very small amounts of RNA (<50 ng). Total RNA was extracted with the use of Qiagen RNeasy Mini Kits for cells and with the use of TRIzol (Invitrogen) for tissue samples according to the manufacturer's protocol. The cDNA was then synthesized with an Omniscript Reverse Transcription (RT) Kit (Qiagen) or Moloney Murine Leukemia Virus Reverse Transcriptase Reverse Transcriptase (M-MLV RT). A high affinity for RNA allows Omniscript Reverse Transcriptase to provide superior performance compared with other reverse transcriptases, delivering higher sensitivity in RT-PCR, even with low. For example, performance of OmniScript and SensiScript (both Qiagen) or AMV-derived RTases have been heavily influenced by experimental. High cDNA yields due to high-affinity enzyme Sensitive detection of as few as 10 copies of template No additional RNase H digestion step required Fast and easy procedure with no tedious pipetting steps The Omniscript Reverse Transcription (RT) Kit is specially designed for reverse transcription with. 95°C after the reverse transcription step. Place a nuclease-free PCR tube on ice and add approximately 2 μg of isolated total RNA. There-fore, 2,500 copies (1 fg) was chosen as the relatively small. Genomic DNA contamination in RNA samples is effectively. PrimeScript RT Reagent Kit is designed to perform reverse transcription optimized for real-time RT-PCR. ers and the same RT kit. ZERO BIAS - scores, article reviews, protocol. The PCR products were resolved by electrophoresis on 1% agarose gels and visualized by ethidium bromide staining. RT-qPCR was carried out inRNA concentration was photometrically determined, and 200 ng RNA was reverse transcribed using Omniscript RT kit with oligo dT Primer (Qiagen, Hilden, Germany). Download. For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRBalanced mixture of enzymes with optimized reverse-transcription buffer The QIAGEN One-Step RT-PCR Kit provides a blend of Sensiscript and Omniscript Reverse Transcriptases, HotStarTaq DNA Polymerase, QIAGEN OneStep RT-PCR Buffer, a dNTP mix, and Q-Solution, a novel additive that enables efficient amplification of “difficult” (e. V. Add 2 μl of Buffer RT (component of Omniscript RT Kit), 2 μl of dNTP Mix (component of. was reverse transcribed to cDNA using the Omniscript RT kit (Qiagen) and random hexamer primers (Invitrogen; Ta˚strup, Denmark). 1 ml RNase-free water 205111 询价Currently, almost all single-cell transcriptomic protocols rely on reverse transcription (RT). Total RNA was isolated from drug-treated cells with TRIzol (Invitrogen) and then reverse-transcribed to cDNA using the Omniscript RT kit (Qiagen, Valencia, CA). One-Step RT-PCR; Reverse Transcription & cDNA Synthesis; Real-Time PCR Enzymes & Kits. Average lead time: Call for Lead Time. The unique combination of Omniscript and Sensiscript Reverse Transcriptases, with their high affinity for RNA templates, ensures highly efficient and sensitive transcription of RNA amounts from as little as 1 pg up to 2 µg. SYBR Green (DyNAmo SYBR Green qPCR Kit; Finnzymes, Finland) and related accessories to perform real-time RT-qPCR (Opticon2; MJ Research, Watertown,. Popular answers (1) "If the mRNA has a poly-A 3' tail, then an oligo-dT primer can be used to prime all mRNAs simultaneously. AIV genomes were amplified from extracted RNA using universal and gene-specific primers with an Omniscript reverse-transcription kit (QIAGEN, Germantown,. Omniscript is designed for reverseFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRThis study provides a complex overview of the influence of elements such as RT systems, qRTPCR chemistry, amount of background RNA, and transcript abundance on the efficiency of qRT-PCR. PCR was performed on an ABI 7900HT using TaqMan® Universal PCR Master Mix, No Amperase® UNG (Life Technologies, Carlsbad, CA) or. High cDNA yields due to high-affinity enzyme Sensitive detection of as few as 10 copies of template No additional RNase H digestion step required Fast and easy procedure with no tedious pipetting steps The Omniscript Reverse Transcription (RT) Kit is specially designed for reverse transcription with. Probe-Based One-Step qRT-PCR; Probe-Based qPCR; Reverse Transcription & cDNA Synthesis for qPCR; SYBR Green- or Dye-Based One-Step qRT-PCR; SYBR Green- or Dye-Based qPCR; qPCR Assays & Instruments. The Omniscript Reverse Transcription (RT) Kit is specially designed for reverse transcription with any amount of RNA from 50 ng to 2 μg per reaction. The RNA yield and quality were determined using a NanoDrop system (ND-1000; Thermo Fisher Scientific). For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRHigh cDNA yields due to high-affinity enzyme Sensitive detection of as few as 10 copies of template No additional RNase H digestion step required Fast and easy procedure with no tedious pipetting steps The Omniscript Reverse Transcription (RT) Kit is specially designed for reverse transcription with. Reverse transcriptase is a multifunctional enzyme with three distinct enzymatic activities: an RNA-dependent DNA polymerase, a hybrid-dependent exoribonuclease (RNase H), and a DNA-dependent DNA polymerase. This kit is ideal for analyzing low input RNA samples. 0 µl 5 mM dNTP (final concentration of 500 µM), 2. ZERO BIAS - scores, article reviews, protocol conditions and moreFor 50 reverse-transcription reactions: 200 units Omniscript Reverse Transcriptase, 150 µl 10x Buffer RT, 100 µl dNTP Mix (contains 5 mM each dNTP), 1. A 20 μl RT reaction included 2 μg Universal reference RNA (Stratagen), 1 μM Oligo-dT primer, 2 μl of 10× RT buffer, 0. My QIAGEN. Kim et al. The QIAGEN OneStep Ahead RT-PCR Kit is designed for easy and sensitive one-step RT-PCR using any RNA template. Unit definition: 2 Products found Omniscript RT Kit For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCR Sensiscript RT Kit For reverse transcription of less than 50 ng RNA per reaction for end-point PCR Discover the products and protocols you need Plan your entire experiment based on research interests. Business Development. RT-PCR stands for Reverse Transcription PCR or Reverse Transcriptase PCR. PCR primers were designed using online available primer design software from Thermo Fisher Scientific and were purchased from Thermo Fisher Scientific. Get Adobe Reader. Real-time PCR analysisReverse Transcriptase was performed using Omniscript RT kit (Qiagen, Valencia, CA, #205113) according to manufacturer’s protocol. Investor Relations Contacts. Omniscript® Reverse Transcriptaseis designed for efficient and sensitive reverse transcription with 50 ng – 2 µg RNA. A high affinity for RNA allows Omniscript Reverse Transcriptase to provide superior performance compared with other reverse transcriptases, delivering higher sensitivity in RT-PCR, even with low. For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCROmniscript Reverse Transcription Handbook 05/2004. Additional Information. First-strand cDNA synthesis was done using the Omniscript RT kit (Qiagen, Valencia, CA) as recommended by the manufacturer. SuperScript II, AMV-RT, Transcriptor RT, M-MuLV RT, M-MLV RT, Omniscript, DyNAmo, Statascript: Covariances of the RT efficiency were driven by target gene or total RNA concentration: Enzyme differences less important than diversity in gene-specific RT reproducibility: Bustin et al. In order to measure this difference on a large spectrum of background RNA, given that DNA and RNA concentrations are important influencing factors [ 8 – 10 ], the study used total RNA extracted from the same testis tissue (Methods). 1 ml 1. This kit's protocol is simple and suitable for high throughput. For reverse transcription of 50 ng to 2 micro g RNA per reaction for end-point PCR. Subsequent PCR was performed with primers (Sigma-Aldrich) based on the Icelandic rock ptarmigan genome and Taq DNA polymerase (QIAGEN). Vlocity Cards Framework. A PCR was conducted using standard PCR methods (primers are available on request). Reverse Transcription Troubleshooting Guide. Need Assistance?. OmniScripts allow you to craft dynamic customer. RT-PCR Assays. For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRSensiscript Reverse Transcriptase is specially designed for highly sensitive reverse transcription with small amounts of RNA (<50 ng). 1 ml RNase-free water 205111 询价For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRHigh cDNA yields due to high-affinity enzyme Sensitive detection of as few as 10 copies of template No additional RNase H digestion step required Fast and easy procedure with no tedious pipetting steps The Omniscript Reverse Transcription (RT) Kit is specially designed for reverse transcription with. For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRNxtScript 2G RT is the latest member of the family and pushes the temperature of the RT step to new levels: no loss of activity even at 70°C. OmniScripts allow you to craft dynamic customer. ) to obtain cDNA. Omniscript reverse transcriptase (QIAGEN) using random hexamers and oligo dT and. , the OmniScript RT, an RNAse H+ RT, which is part of the QuantiTect Reverse Transcription Kit, as well as with RNase H. A high affinity for RNA allows Omniscript Reverse Transcriptase to provide superior performance compared with other reverse transcriptases, delivering higher sensitivity in RT-PCR, even with low. 1 ml 4. Reverse transcription with AMV, MMLV, and Omniscript was performed at 37 °C, whereas with cAMV, Improm-II, and MMLVH it was performed at 45 °C, and with ThermoScript and SuperScript, it was performed at 50 °C. CNV qPCR Assays & Arrays;. g. High Ca2+ stimulated the upregulation (as assessed by RT-PCR) and the secretion of Wnt5a (assessed by Western blot), a. The enzyme mix is specially formulated for both reverse transcription and PCR. USD $1207. Data Migration with Build Tool. Omniscript RT system, whereas substantial copies of. The Omniscript Reverse Transcription (RT) Kit is specially designed for reverse transcription with any amount of RNA from 50 ng to 2 μg per reaction. For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRQuantiTect RT Mix contains an optimized Omniscript and Sensiscript blend. For 200 reverse-transcription reactions: 800 units Omniscript Reverse Transcriptase, 4 x 150 µl 10x Buffer RT, 4 x 100 µl dNTP Mix (contains 5 mM each dNTP), 4 x 1. A high affinity for RNA allows Omniscript Reverse Transcriptase to provide superior performance compared with other reverse transcriptases, delivering higher sensitivity in RT-PCR, even with low. This kit's protocol is simple and suitable for high throughput. Total RNA from flag leaves of mature rice plants was isolated using the RNeasy Plant Mini Kit (Qiagen, Venlo, The Netherlands) and reversely transcribed using Omniscript RT Kit (Qiagen). Omniscript RT system, whereas substantial copies of. For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCROne-Step RT-PCR; Reverse Transcription & cDNA Synthesis; Real-Time PCR Enzymes & Kits. However, RT is recognized as a known source of variability, particularly with low amounts of RNA. Data Migration with Build Tool. It allows for the efficient labeling of a wide range of starting RNA concentrations and. , Valencia, CA). 5 mM each dNTP, 10 U of RNase inhibitor, 4 U of Omniscript Reverse Transcriptase, and. The Omniscript Reverse Transcription (RT) Kit is specially designed for reverse transcription with any amount of RNA from 50 ng to 2 μg per reaction. Mar 15, 2021. For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFAQ. Note: Commercially available RNase inhibitor is commonly supplied at 40 units/µl. A high affinity for RNA allows Omniscript Reverse Transcriptase to provide superior performance compared with other reverse transcriptases, delivering higher sensitivity in RT-PCR, even with low. Please see the Omniscript Reverse Transcription Handbook for protocols. A high affinity for RNA allows Omniscript Reverse. 1 ml RNase-free water 205111 询价 For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCR High cDNA yields due to high-affinity enzyme Sensitive detection of as few as 10 copies of template No additional RNase H digestion step required Fast and easy procedure with no tedious pipetting steps The Omniscript Reverse Transcription (RT) Kit is specially designed for reverse transcription with. 00. RT reactions containing 4 ng (Sensiscript) or 16 ng of total RNA (Omniscript) generated DD patterns indistinguishable from the 200 ng pattern. For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCROne-Step RT-PCR; Reverse Transcription & cDNA Synthesis; Real-Time PCR Enzymes & Kits. Probe-Based One-Step qRT-PCR; Probe-Based qPCR; Reverse Transcription & cDNA Synthesis for qPCR; SYBR Green- or Dye-Based One-Step qRT-PCR; SYBR Green- or Dye-Based qPCR; qPCR Assays & Instruments. For 50 reverse-transcription reactions: 200 units Omniscript Reverse Transcriptase, 150 µl 10x Buffer RT, 100 µl dNTP Mix (contains 5 mM each dNTP), 1. PCR and RT-PCR are achieved by the use of the hot-start enzyme HotStarTaq Plus DNA Polymerase together with a specialized fast PCR or RT-PCR buffer. OmniScript にはモジュール形式のアーキテクチャが採用されている OmniScript の外観 (フロントエンド) は機能 (バックエンド) から切り離されています。 OmniScript では JavaScript Object Notation (JSON) メタデータ構造 (1)、スタイルシート (2)、データ (3) が互いに分離され. Bioz Stars score: 86/100, based on 1 PubMed citations. However, the Sensiscript and PowerScript systems were more efficient for detecting high. Omniscript Reverse Transcriptase - For First-strand cDNA synthesis Two-tube RT-PCR. A unique enzyme combination and specially developed reaction buffer ensure efficient, highly specific reverse transcription and PCR in one tube, without the need for. Omniscript is designed for reverse transcription of RNA amounts greater than 50 ng, andFor reverse transcription of 50 ng to 2 μg RNA per reaction fork end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRExperimental validation. 1 ml 1. A high affinity for RNA allows Omniscript Reverse Transcriptase to provide superior performance compared with other reverse transcriptases, delivering higher sensitivity in RT-PCR, even with low. Probe-Based One-Step qRT-PCR; Probe-Based qPCR; Reverse Transcription & cDNA Synthesis for qPCR; SYBR Green- or Dye-Based One-Step qRT-PCR; SYBR Green- or Dye-Based qPCR; qPCR Assays & Instruments. DSL Modem has an attribute Grade (ATT_RT_GR) and possible values are Good, Better and Best. RT activity is critical for many basic techniques including real-time and endpoint RT-PCR, labeled-probe generation for microarrays and cDNA cloning. The maximum amount of viral RNA was used in place of water for both reactions. 2) If you only wanted to produce cDNA from a subset of all mRNA, then. OmniScripts are built by moving elements into the canvas and defining their properties. For reverse transcription from 50 ng to 2 μg RNA per reaction for end-point PCRAdditional Information. The Omniscript RT Kit is intended for molecular biology applications. 205110 205111 205113 Number of reactions* 10 50 200 Omniscript Reverse Transcriptase 40 units 200 units 800 units Buffer RT, 10x 30 µl 150 µl 4 x 150 µl dNTP Mix, 5 mM each 20 µl 100 µl 4 x 100 µl RNase-Free Water 1. For MMLV Superscript, the pattern obtained was very faint at 200 ng of RNA, but indicated a >50-fold increase in detection levels for Sensiscript. For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRThe Omniscript Reverse Transcription (RT) Kit is specially designed for reverse transcription with any amount of RNA from 50 ng to 2 μg per reaction. Kit contents: Qiagen Omniscript RT Kit, 50 rxns, RNA Template Sample, Reverse Transcription Enzyme Activity, High cDNA Yields Due to High-affinity Enzyme, Fast and Easy Procedure, For Reverse Transcription of 50ng to 2micro g RNA/Reaction for end-point PCR, Includes 200U Omniscript Reverse. One microliter of kit-supplied enzyme mix-ture (combination of Omniscript Reverse. This product is not intended for the diagnosis, prevention, or treatment of a disease. IL-10 was demonstrated to be critical for suppressing autoimmunity and inflammatory responses ( 40 – 43 ), and whether Th17 express IL-10 is. Two micrograms of total RNA was reverse-transcribed using an Omniscript Reverse Transcriptase Kit (Qiagen) in a total volume of 20 µl. 0 µl 10× RT buffer, 2. For standard reverse transcription, with 50 ng to 2 µg of RNA per reaction, Omniscript RT provides optimal results for both high- and low-copy mRNA species. Bioz Stars score: 86/100, based on 1 PubMed citations. The Omniscript Reverse Transcription (RT) Kit is specially designed for reverse transcription with any amount of RNA from 50 ng to 2 μg per reaction. Global contacts. (2010). Quantitative RT-PCR Analyses Total RNA of the collected samples were extracted using the RNeasy Plant Mini Kit (Qiagen, Hilden, Germany). Sample to Insight__ Quick-Start Protocol March 2016 Omniscript® Reverse Transcription Kit The Omniscript RT Kit (cat. PCR was performed with HotStarTaq PCR Kit (Qiagen). RNA was the same set of samples used for Illumina GAII sequencing analysis. Please see the Omniscript Reverse Transcription Handbook for protocols. Both enzymes exhibit a high affinity for RNA, facilitating transcription through secondary structures that may inhibit other reverse transcriptases. For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRThe reverse transcription was achieved using Omniscript RT Kit (Qiagen), according to manufacturer’s instructions. One-Step RT-PCR; Reverse Transcription & cDNA Synthesis; Real-Time PCR Enzymes & Kits. Omniscript is a confident hand-lettered script without self-conscious style or idiosyncrasy. Matrix assay. A total of 1. First-strand cDNA was synthesized from 1 μg of high-quality total RNA using SuperScript II Reverse Transcriptase in accordance with the manufacturer’s instructions (QIAGEN, Omniscript RT). For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor RNA, is there a difference in selecting RT enzymes with and without RNase H (e. Probe-Based One-Step qRT-PCR; Probe-Based qPCR; Reverse Transcription & cDNA Synthesis for qPCR; SYBR Green- or Dye-Based One-Step qRT-PCR; SYBR Green- or Dye-Based qPCR; qPCR Assays & Instruments. Probe-Based One-Step qRT-PCR; Probe-Based qPCR; Reverse Transcription & cDNA Synthesis for qPCR; SYBR Green- or Dye-Based One-Step qRT-PCR; SYBR Green- or Dye-Based qPCR; qPCR Assays & Instruments. 2 Products found Omniscript RT Kit For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCR Sensiscript RT Kit For reverse transcription of less than 50 ng RNA per reaction for end-point PCR. The ideal reverse transcriptase is robust (highly active under a variety of conditions) and converts all primed RNA within a sample to cDNA, regardless of its abundance, length or secondary structure. 1 ml 1. 1fg/µl). Life Science Grants. For 200 reverse-transcription reactions: 800 units Omniscript Reverse Transcriptase, 4 × 150 µL 10 × Buffer RT, 4 × 100 µL dNTP Mix (contains 5 mM each dNTP), 4 × 1. RT-PCR Analysis . Global Contacts. For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRReal-time RT-PCR A OneStep RT-PCR Kitb was used with a 25-#l reaction volume. Please see the Omniscript Reverse Transcription Handbook for protocols. 205110 205111 205113 Number of reactions* 10 50 200 Omniscript Reverse Transcriptase 40 units 200 units 800 units Buffer RT, 10x 30 µl 150 µl 4 x 150 µl dNTP Mix, 5 mM each 20 µl 100 µl 4 x 100 µl RNase-Free Water 1. Total RNA was reverse-transcribed to cDNA with the use of Qiagen Omniscript RT Kit at 37°C for 60 minutes. For one-step RT-PCR, the optimized Omniscript and Sensiscript blend for the reverse-transcription step further enhances sensitivity. The QIAGEN One-Step RT-PCR Kit provides a blend of Sensiscript and Omniscript Reverse Transcriptases, HotStarTaq DNA Polymerase, QIAGEN OneStep RT-PCR. A high affinity for RNA allows Omniscript Reverse Transcriptase to provide superior performance compared with other reverse transcriptases, delivering higher sensitivity in RT-PCR, even with low. A high affinity for RNA allows Omniscript Reverse Transcriptase to provide superior performance compared with other reverse transcriptases, delivering higher sensitivity in RT-PCR, even with low. Taqman primers of selectin E. For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCR4 Omniscript Reverse Transcription Handbook 10/2010 Kit Contents Omniscript RT Kit (10) (50) (200) Catalog no. The Omniscript Reverse Transcription (RT) Kit is specially designed for reverse transcription with any amount of RNA from 50 ng to 2 μg per reaction. For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRLaunching an OmniScript with an OS Action in a Community; Launching an OmniScript from a Layout Action; Launching an OmniScript with an OS Action in Lightning; Launching an LWC OmniScript in Lightning; Launching an OmniScript in Lightning; Launching an OmniScript From the Action Toolbar; Launching an LWC OmniScript from an OS. 1 ml RNase-free water 205111 询价 The Omniscript RT Kit includes Omniscript Reverse Transcriptase, dNTPs and an optimized reaction buffer. Semiquantitative RT-PCR. It uses PrimeScript Reverse Transcriptase, which features excellent extension. High cDNA yields due to high-affinity enzyme Sensitive detection of as few as 10 copies of template No additional RNase H digestion step required Fast and easy procedure with no tedious pipetting steps The Omniscript Reverse Transcription (RT) Kit is specially designed for reverse transcription with. The commercial RT systems tested were Sensiscript, Omniscript, SuperScript II, SuperScript III, and PowerScript. The ABScript II Reverse Transcriptase in. IVDR Support. The Omniscript RT Kit includes Omniscript Reverse Transcriptase, dNTPs and an optimized reaction buffer. File size. The Omniscript Reverse Transcription (RT) Kit is specially designed for reverse transcription with any amount of RNA from 50 ng to 2 μg per reaction. show that ferrets are highly susceptible to SARS-CoV-2 infection and effectively transmit the virus by direct or indirect contact, recapitulating human infection. Relative quantification of mRNA levels by RT-qPCR was performed using a SYBR Green PCR kit (Qiagen Inc. EGFP cDNA were obtained with th e other RTases. OmniScript は、お客様がビジネスプロセスを完了するためのガイド付きパスを提供し、シームレスなカスタマーエクスペリエンス (これは 常に 好ましいことです) を実現するための設定可能な方法として機能します。. using an Omniscript reverse transcriptase kit (Qiagen, Valencia, CA) in a 20- l reaction mixture, containing 200 ng of total RNA and specific primers, at 37°C for 1 h. At higher temperatures, RNA secondary structures unravel and no longer. The mRNA levels were quantified using a SYBR Premix Ex Kit (Bio‑Rad) with a CFX Real time PCR Detection Systems (Bio‑Rad). The reverse transcription (RT) step conditions for all primer sets were 30 min at 50 C and 15 min at 95 C. Quantitative real-time PCR (qPCR) was performed using the SYBR Premix Ex Taq (TaKaRa, Dalian, China) and the CFX96 TM Real-Time PCR. Please see the Omniscript Reverse Transcription Handbook for protocols. Simply add primers for fast and easy cDNA synthesis. For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRThe Omniscript Reverse Transcription (RT) Kit is specially designed for reverse transcription with any amount of RNA from 50 ng to 2 μg per reaction. For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCROmniscript Quantiscript 37 NA mRNA Random hexamers Paulin et al. coli. High cDNA yields due to high-affinity enzyme Sensitive detection of as few as 10 copies of template No additional RNase H digestion step required Fast and easy procedure with no tedious pipetting steps The Omniscript Reverse Transcription (RT) Kit is specially designed for reverse transcription with. For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCR Enzyme activity: Reverse tran…The Sensiscript Reverse Transcriptase (RT) Kit is ideally suited for highly sensitive applications using very small amounts of RNA (less than 50. 在终点法PCR中，用于RNA模板量为50 ng到2 μg的逆转录反应For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCR高感度リアルタイム2ステップRT-PCRによる遺伝子発現解析 でcDNA合成とgDNA除去をわずか20分で実現する高速 cDNA合成のために、 5'および3'の転写産物の少ないcDNA合成でも高いcDNA収量 を実現。 RNA特異的なプライマーやプローブ ユニークなQuantiTect逆転写キットは. RT activity is critical for many basic techniques including real-time and endpoint RT-PCR, labeled-probe generation for microarrays and cDNA cloning. The rela‑ tive gene expression levels were normalized to GAPDH and calculated using the 2‑ΔΔCq method. 1 ml 4. For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor standard reverse transcription, with 50 ng to 2 µg of RNA per reaction, Omniscript RT provides optimal results for both high- and low-copy mRNA species. A high affinity for RNA allows Omniscript Reverse Transcriptase to provide superior performance compared with other reverse transcriptases, delivering higher sensitivity in RT-PCR, even with low. Inquiry. Genomic DNA contamination in RNA samples is effectively eliminated by gDNA Wipeout Buffer. During reverse transcription, chemically modified HotStarTaq DNA Polymerase is completely inactive and does not interfere with the reverse-transcriptase reaction. In brief, 2 μg of RNA were incubated with 1 μg of Oligo-dT primers in the presence of 500 nmol/L deoxynucleotide triphosphate, 10 units of RNase inhibitor (Qiagen), and 10 units of Omniscript RT in 1× reverse. Heat-mediated activation of these enzymes enables reaction setup at room temperature. It uses PrimeScript Reverse Transcriptase, which features excellent extension. Components. less than 500 ng total RNA). There are several commercially available RT kits which differ mainly in the kind of enzyme they use, bacterial (rTth) or viral origin (e. QIAstock. Both. ). It is also nice that the purification procedure (after labeling) is simple. 1 mL RNase-free. 4 Omniscript Reverse Transcription Handbook 10/2010 Kit Contents Omniscript RT Kit (10) (50) (200) Catalog no. English. 1 ml 4. The ABScript II One Step RT-qPCR Probe Kit is a convenient, ready-to-use kit with optimized components for use for reverse transcription and subsequent probe-based qPCR. Reverse Transcription Kit (Omniscript® RT Kit) as per the manufacturer's instructions (Qiagen). RNA was transcribed into cDNA using the Omniscript RT kit (Qiagen). Probably the most important being that the OmniScript RT allows for the labeling of even very small amounts of RNA (i. For 50 reverse-transcription reactions: 200 units Omniscript Reverse Transcriptase, 150 µl 10x Buffer RT, 100 µl dNTP Mix (contains 5 mM each dNTP), 1. 205110 205111 205113 Number of reactions* 10 50 200 Omniscript Reverse Transcriptase 40 units 200 units 800 units Buffer RT, 10x 30 µl 150 µl 4 x 150 µl dNTP Mix, 5 mM each 20 µl 100 µl 4 x 100 µl RNase-free water 1. An OmniScript is an omnichannel customer engagement and a business tool built on the Salesforce platform. Background Reverse transcription-polymerase chain reaction (RT-PCR) is a very sensitive technique to measure and to compare mRNA levels among samples. Sensiscript Reverse Transcriptase is specially designed for highly sensitive reverse transcription with small amounts of RNA (<50 ng). This kit is ideal for analyzing low input RNA samples. PCR reactions were performed to determine Clk 1 mRNA levels using JumpStart Ready-Start amplification mix (Sigma) and previously published primers. A Vlocity OmniScript guides users through complex processes with fast, personalized, and consistent responses. nos. For MMLV Superscript, the pattern obtained was very faint at 200 ng of RNA, but indicated a >50-fold increase in detection levels for Sensiscript. 1 ml 1. The easy one-tube setup and optimized components enable higher sensitivity. The ABScript II Reverse Transcriptase in the. For reverse transcription of 50 ng to 2 μg RNA per reaction fork end-point PCR Omniscript Reverse Transcription（RT）Kitは、各反応あたり50 ngから2 µgのRNA量を用いた逆転写用に特別にデザインされています。 RNAへのアフィニティーが高いOmniscript Reverse Transcriptaseは、他の逆転写酵素と比較して非常に優れた性能を示し、低いコピー数の. g. For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRReverse transcription was carried out according to the manufacturer's protocol either with the Omniscript RT (Qiagen) or SuperScript II RT (Invitrogen). Pharmaceutical Industry. この例には、再利用可能な. Probe-Based One-Step qRT-PCR; Probe-Based qPCR; Reverse Transcription & cDNA Synthesis for qPCR; SYBR Green- or Dye-Based One-Step qRT-PCR; SYBR Green- or Dye-Based qPCR; qPCR Assays & Instruments. 05). Reverse transcription was applied with Omniscript RT Kit following manufacturer's protocol (Qiagen). Reactions containing 2. OmniScripts 要素 (子 OmniScript) は、OmniScript をコールして別の OmniScript 内で再利用します。. Time step T0 contained the reverse transcription mix (Omniscript RT Kit) without performing reverse transcription, T1 after the reverse transcription, and T2 was after a multiple displacement. The commercial RT systems tested were Sensiscript, Omniscript, SuperScript II, SuperScript III, and PowerScript. For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRHigh cDNA yields due to high-affinity enzyme Sensitive detection of as few as 10 copies of template No additional RNase H digestion step required Fast and easy procedure with no tedious pipetting steps The Omniscript Reverse Transcription (RT) Kit is specially designed for reverse transcription with. Omniscript RT Kit. Two micrograms of total RNA was reverse-transcribed using Omniscript RT kit (Qiagen). 2) If you only wanted to produce cDNA from a subset of all mRNA, then. OmniScript にはモジュール形式のアーキテクチャが採用されている OmniScript の外観 (フロントエンド) は機能 (バックエンド) から切り離されています。 OmniScript では JavaScript Object Notation (JSON) メタデータ構造 (1)、スタイルシート (2)、データ (3) が互いに分離され. Differences Between OmniStudio and OmniStudio for Vlocity. 1 mL RNase-free water. 1 ml 4. 381KB. Key words: Breast cancer, Formalin-fixed, Paraffin-embedded tissue, Quantitative RT-PCR,Reverse Transcription was performed using an OmniScript™ RT kit (QIAGEN) according to manufacturer’s instructions and using gene-specific primers to interrogate genes of interest. CNV qPCR Assays & Arrays;. Reverse transcription with AMV, MMLV, and Omniscript was performed at 37 °C, whereas with cAMV, Improm-II, and MMLVH it was performed at 45 °C, and with ThermoScript and SuperScript, it was performed at 50 °C. Omniscript® Reverse Transcriptaseis designed for efficient and sensitive reverse transcription with 50 ng – 2 µg RNA. For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCROne-Step RT-PCR; Reverse Transcription & cDNA Synthesis; Real-Time PCR Enzymes & Kits. The assays were run under the following conditions. The QIAGEN OneStep RT-PCR Enzyme Mix contains a specially formulated enzyme blend for both reverse transcription and PCR. 205110 205111 205113 Number of reactions* 10 50 200 Omniscript Reverse Transcriptase 40 units 200 units 800 units Buffer RT, 10x 30 µl 150 µl 4 x 150 µl dNTP Mix, 5 mM each 20 µl 100 µl 4 x 100 µl RNase-Free Water 1. The sequences of the primers used in. 1 ml 4. The unique QuantiTect Reverse Transcription Kit provides a fast and convenient procedure for cDNA synthesis with integrated genomic DNA removal. CNV qPCR Assays & Arrays; Microbial DNA qPCR Assays & Panels For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCR For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCR Qiagen omniscript rt kit Omniscript Rt Kit, supplied by Qiagen, used in various techniques. High yields of cDNA 특이성을 높인 참신한 enzyme과 buffer No additional RNase H digestion step required Robust system guarantees consistent results The Omniscript Reverse Transcription (RT) Kit is specially designed for reverse transcription with any amount of RNA from 50 ng to 2 μg per. これにより、特定の取引先責任者や取引先の項目を表示するなどの一般的な機能を持つ小さな「子」 OmniScript を作成することができます。. Both biological and technical triplicates were run. CNV qPCR Assays & Arrays;. icon_0011_idea-s. For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCR The Omniscript Reverse Transcription (RT) Kit is specially designed for reverse transcription with any amount of RNA from 50 ng to 2 μg per reaction. Bioz Stars score: 96/100, based on 1 PubMed citations. RT-PCR. With no need for optimization, the Omniscript RT Kit makes reverse transcription virtually trouble-free. Content. Omniscript RT Kit (200) by Qiagen. CNV qPCR Assays & Arrays;. For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCROmniScripts. A high affinity for RNA allows Omniscript Reverse Transcriptase to provide superior performance compared with other reverse transcriptases, delivering higher sensitivity in RT-PCR, even with low. Product tracking (in bp) are shown about the right of the panel (lane 9). Migrate to Standard OmniStudio. All seven samples were reverse transcribed by the same kit toFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRHigh cDNA yields due to high-affinity enzyme Sensitive detection of as few as 10 copies of template No additional RNase H digestion step required Fast and easy procedure with no tedious pipetting steps The Omniscript Reverse Transcription (RT) Kit is specially designed for reverse transcription with. 1 ml 1. For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRSensiscript Reverse Transcriptase is specially designed for highly sensitive reverse transcription with small amounts of RNA (<50 ng). ZERO BIAS - scores, article reviews, protocol conditions and more For 50 reverse-transcription reactions: 200 units Omniscript Reverse Transcriptase, 150 µl 10x Buffer RT, 100 µl dNTP Mix (contains 5 mM each dNTP), 1. This also inactivates the reverse transcriptases. 1. Omniscript RT Kit. Kit contents: Qiagen Omniscript RT Kit, 200 rxns, RNA Template Sample, Reverse Transcription Enzyme Activity, High cDNA Yields Due to High-affinity Enzyme, Fast and Easy Procedure, For Reverse Transcription of 50ng to 2micro g. The Omniscript Reverse Transcription (RT) Kit is specially designed for reverse transcription with any amount of RNA from 50 ng to 2 μg per reaction. QIAGEN Subsidiaries. The Omniscript Reverse Transcription (RT) Kit is specially designed for reverse transcription with any amount of RNA from 50 ng to 2 μg per. For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor backward transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCROne-Step RT-PCR; Reverse Transcription & cDNA Synthesis; Real-Time PCR Enzymes & Kits. , SuperScript vs. We compared these five RTs for their utility in detecting trace transcripts by real-time PCR, ability to transcribe a long RNA and sensitivity. Bioz Stars score: 86/100, based on 1 PubMed citations. QIAsphere Digital Connectivity. # 205113 and 205213, respectively) reverse transcriptases. OmniScript は、お客様がビジネスプロセスを完了するためのガイド付きパスを提供し、シームレスなカスタマーエクスペリエンス (これは 常に 好ましいことです) を実現するため. 1 ml RNase-free water 205111 询价 Reverse transcription and quantitative real-time PCR (RT-qPCR). All reactions took place for 1h at 37°C in a final volume of 40 μl containing 1 μg total RNA in the presence of 20 units RNase inhibitor (RNasin® Ribonuclease Inhibitor, Promega), 1 μg random hexamers (Microsynth), 4 μl 10. Qiagen confirmed that such a plasmid is. Omniscript® Reverse Transcriptaseis designed for efficient and sensitive reverse transcription with 50 ng – 2 µg RNA. $1,382. Format. Omniscript RT Kit. 95°C after the reverse transcription step. Content. Omniscript RT Kit. Omniscript® Reverse Transcriptaseis designed for efficient and sensitive reverse transcription with 50 ng – 2 µg RNA. A high affinity for RNA allows Omniscript Reverse Transcriptase to provide superior performance compared with other reverse transcriptases, delivering higher sensitivity in RT-PCR, even with low. Media Inquiries. Reactions were incubated at 65°C for 5 min, then 100 U RT enzyme was added and incubated at 37°C for 60 min and 75°C for 5 min. The Omniscript Reverse Transcription (RT) Kit is specially designed for reverse transcription with any amount of RNA from 50 ng to 2 μg per reaction. 1 ml RNase-free water 205111 询价The Omniscript Reverse Transcription (RT) Kit is specially designed for reverse transcription with any amount of RNA from 50 ng to 2 μg per reaction. ,. The Omniscript Reverse Transcription (RT) Kit is specially designed for reverse transcription with any amount of RNA from 50 ng to 2 μg per reaction. In a final. ·. For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRHigh cDNA yields due to high-affinity enzyme Sensitive detection of as few as 10 copies of template No additional RNase H digestion step required Fast and easy procedure with no tedious pipetting steps The Omniscript Reverse Transcription (RT) Kit is specially designed for reverse transcription with. 1 ml 4 x. Please see the Omniscript Reverse Transcription Handbook for protocols. For each RTase, the reaction conditions and thermal profile followed the recommended protocol issued by the manufacturer (see Protocols in online Data Supplement 1). Nine parallel large-scale GoScript™ RT reactions were performed, containing log-fold variations of input human reference total RNA (10ng/µl to 0. For reverse transcription of 50 ng to 2 micro g RNA per reaction for end-point PCR. For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRHigh cDNA yields due to high-affinity enzyme Sensitive detection of as few as 10 copies of template No additional RNase H digestion step required Fast and easy procedure with no tedious pipetting steps The Omniscript Reverse Transcription (RT) Kit is specially designed for reverse transcription with. For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRRT reactions containing 4 ng (Sensiscript) or 16 ng of total RNA (Omniscript) generated DD patterns indistinguishable from the 200 ng pattern. Serial analysis of BCR-ABL mRNA levels by real-time quantitative PCR (RQ-PCR) accurately reflects the level of leukemic inhibition induced by therapy and provides.